Around 500 Wilson James Heathrow workers providing assistance to passengers with restricted mobility (PRM) will strike for four days over the Easter holidays.
Unite said the workers are paid an average of 9.5% less than Wilson James staff at Gatwick performing the same role.
The workers will strike from 5th April to 9th April.
Sharon Graham, general secretary at Unite, said: “Wilson James and Heathrow are both highly profitable, so there is absolutely no excuse for these workers to be paid far less than their colleagues at Gatwick.
“Unite is backing these workers 100% in their fight for fair pay.”
Martin West, regional officer at Unite, said: “Wilson James’ Heathrow workers go above and beyond in their work every day to support and look after passengers.
“They care deeply about their jobs but have been forced into taking strike action because Wilson James is treating them as second-class workers.
“The responsibility for the disruption that will be caused lies entirely with the company.
“HAL, which is hugely profitable, needs to step in and ensure Wilson James tables a pay offer that takes into account the indefensible difference in wages between Heathrow and Gatwick.”
Workplace Journal has reached out to Wilson James for a comment.